Symmetry‐breaking in branching epithelia: cells on micro‐patterns under flow challenge the hypothesis of positive feedback by a secreted autocrine inhibitor of motility

Autor:	Martin, Kimberly C., Yuan, Xiaofei, Stimac, Gregory, Bannerman, Kieran, Anderson, Jamie, Roy, Chloe, Glykofrydis, Fokion, Yin, Huabing, Davies, Jamie A.
Jazyk:	angličtina
Rok vydání:	2017
Předmět:	Feedback Physiological autocrine secretion Cell Culture Techniques positive feedback Epithelial Cells Original Articles cell motility shape Autocrine Communication Dogs Cell Movement self‐organisation Animals Original Article branching morphogenesis Cell Shape
Zdroj:	Journal of Anatomy
ISSN:	1469-7580 0021-8782
Popis:	Branching morphogenesis of epithelia involves division of cells into leader (tip) and follower (stalk) cells. Published work on cell lines in culture has suggested that symmetry‐breaking takes place via a secreted autocrine inhibitor of motility, the inhibitor accumulating more in concave regions of the culture boundary, slowing advance of cells there, and less in convex areas, allowing advance and a further exaggeration of the concave/convex difference. Here we test this hypothesis using a two‐dimensional culture system that includes strong flow conditions to remove accumulating diffusible secretions. We find that, while motility does indeed follow boundary curvature in this system, flow makes no difference: this challenges the hypothesis of control by a diffusible secreted autocrine inhibitor.
Databáze:	OpenAIRE
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