| Autor: |
Lenka, Svihálková-Sindlerová, Vendula, Foltinová, Alena, Vaculová, Viktor, Horváth, Karel, Soucek, Petr, Sova, Jirina, Hofmanová, Alois, Kozubík |
| Rok vydání: |
2010 |
| Předmět: |
|
| Zdroj: |
Anticancer research. 30(4) |
| ISSN: |
1791-7530 |
| Popis: |
LA-12 is a new platinum (IV) drug with promising cytotoxic effects in a wide range of cancer cell lines. Its confluence-dependent effects were compared with cisplatin (CDDP) and oxaliplatin (L-OHP) in HT-29 cells.Cytotoxicity was determined by MTT test, eosin exclusion assay, and cell number quantification. The cell cycle was analysed using propidium iodide DNA staining (flow cytometry), apoptosis by phosphatidylserine externalisation (annexin-V assay), mitochondrial membrane potential by flow cytometry, nuclear morphology by means of fluorescence microscopy, and PARP cleavage by Western blotting.While L-OHP and CDDP were practically inactive in the subconfluent cell population, LA-12 showed a similar toxicity in both subconfluent and growing populations. All compounds induced apoptosis, although with different potentials.LA-12 was able to overcome confluence-dependent resistance of HT-29 cells observed for other platinum compounds, which may have potential therapeutic use in slowly growing tumours. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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