| Autor: |
L, Veselský, D, Cechová, M, Hosková, J, Stédra, V, Holáñ, R, Stanék |
| Rok vydání: |
1991 |
| Předmět: |
|
| Zdroj: |
International journal of fertility. 36(3) |
| ISSN: |
0020-725X |
| Popis: |
Boar seminal vesicle fluid inhibits blast transformation of porcine lymphocytes. Boar seminal vesicle fluid was precipitated in 8% ethanol and the dissolved precipitate separated into two peaks upon chromatography on a Sephacryl S-200 column. The inhibitory activity was found predominantly in the second peak. This peak was rechromatographed on the Sephacryl S-200 column, and the fractions with inhibitory activity were pooled and passed down a Sephadex G-75 column. This chromatography run separated the accompanying protease inhibitor from the immunosuppressive fraction. The molecular mass, estimated by chromatography on the Sephadex G-75 column, was 25,000-27,000 for the immunosuppressive factor and 7,500 for the protease inhibitor. Activity for the immunosuppressive fraction was also demonstrated in vivo. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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