| Autor: |
W S, Dynan, J J, Jendrisak, D A, Hager, R R, Burgess |
| Rok vydání: |
1981 |
| Předmět: |
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| Zdroj: |
The Journal of biological chemistry. 256(11) |
| ISSN: |
0021-9258 |
| Popis: |
Wheat germ contains an enzyme capable of removing supercoils from circular DNA. We have purified this enzyme using Polymin P fractionation, ammonium sulfate precipitation, and chromatography on Bio-Rex 70 and phenyl-Sepharose. Renaturation after electrophoresis on sodium dodecyl sulfate-polyacrylamide gels shows that topoisomerase activity is associated with a polypeptide with a Mr = about 111,000. The enzyme is similar to other eukaryotic type I DNA topoisomerases (nicking-closing enzymes) by the following criteria: it is capable of increasing or decreasing the topological linking number of covalently closed DNA substrate; it is capable of restoring an equilibrium distribution of linking numbers to DNA substrate with a single unique linking number; and it does not require magnesium ion or ATP for activity. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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