SIR2 and other genes are abundantly expressed in long-lived natural segregants for replicative aging of the budding yeast Saccharomyces cerevisiae
| Autor: | Zhenhua, Guo, Aleksandra B, Adomas, Erin D, Jackson, Hong, Qin, Jeffrey P, Townsend |
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| Rok vydání: | 2011 |
| Předmět: |
Microbial Viability
Saccharomyces cerevisiae Proteins Gene Expression Profiling Cell Cycle Genes Fungal HSP30 Heat-Shock Proteins Hydrogen Peroxide Saccharomyces cerevisiae Mitochondrial Membrane Transport Proteins Polymerase Chain Reaction Up-Regulation Phenotype Sirtuin 2 Gene Expression Regulation Fungal Mitochondrial Precursor Protein Import Complex Proteins Silent Information Regulator Proteins Saccharomyces cerevisiae Cell Proliferation Oligonucleotide Array Sequence Analysis |
| Zdroj: | FEMS yeast research. 11(4) |
| ISSN: | 1567-1364 |
| Popis: | We investigated the mechanism underlying the natural variation in longevity within natural populations using the model budding yeast, Saccharomyces cerevisiae. We analyzed whole-genome gene expression in four progeny of a natural S. cerevisiae strain that display differential replicative aging. Genes with different expression levels in short- and long-lived strains were classified disproportionately into metabolism, transport, development, transcription or cell cycle, and organelle organization (mitochondrial, chromosomal, and cytoskeletal). With several independent validating experiments, we detected 15 genes with consistent differential expression levels between the long- and the short-lived progeny. Among those 15, SIR2, HSP30, and TIM17 were upregulated in long-lived strains, which is consistent with the known effects of gene silencing, stress response, and mitochondrial function on aging. The link between SIR2 and yeast natural life span variation offers some intriguing ties to the allelic association of the human homolog SIRT1 to visceral obesity and metabolic response to lifestyle intervention. |
| Databáze: | OpenAIRE |
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