| Autor: |
F, Coudore, D, Ardid, A, Eschalier, J, Lavarenne, J, Fialip |
| Rok vydání: |
1992 |
| Předmět: |
|
| Zdroj: |
Journal of chromatography. 584(2) |
| Popis: |
A method was developed for the assay of amitriptyline, amitriptyline N-oxide, nortriptyline, desmethylnortriptyline and E (trans) and Z (cis) isomers of 10-hydroxyamitriptyline and of 10-hydroxynortriptyline in plasma and brain of animals, using high-performance liquid chromatography with ultraviolet detection (254 nm). Single extraction was performed at pH 10.5 from 0.25 ml of plasma or 1 ml of brain mixture. Chromatographic separations were achieved with a silica column and an aqueous methanol mobile phase containing ammonia. This procedure offers high sensitivity (8-10 ng/ml), high linearity (r0.99) and acceptable precision (coefficient of variationor = 13.3%). The method was used to determine levels of amitriptyline and its major metabolites in mice 30 min after a single intraperitoneal administration of amitriptyline (20 mg/kg). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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