LPS-induced inflammation delays the transportation of ASP
| Autor: | Dalang, Li, Chuanzong, Qi, Jian, Zhou, Zeqiang, Wen, Xiangyu, Zhu, Hongguang, Xia, Jue, Song |
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| Rok vydání: | 2019 |
| Předmět: |
Inflammation
Lipopolysaccharides Organic Cation Transport Proteins Symporters Interleukin-6 Tumor Necrosis Factor-alpha Interleukin-1beta Interleukin-18 Down-Regulation Pyridinium Compounds A549 Cells Alveolar Epithelial Cells Cell Line Tumor Humans Solute Carrier Family 22 Member 5 Lung Fluorescent Dyes |
| Zdroj: | Journal of drug targeting. 28(4) |
| ISSN: | 1029-2330 |
| Popis: | Organic cation transporters (OCTNs) can significantly affect drug disposition in alveolar epithelial cells (A549), but this process is not well understood. We investigated the expression and function of OCTN1/2 in A549 cells under different inflammatory status to examine pulmonary drug distribution. This experiment used lipopolysaccharide (LPS)-treated A549 cells to mimic inflammation in alveolar epithelial cells, and the expression of OCTN1/2, interleukin-6 (IL6), IL18, IL1β and tumour necrosis factor-alpha (TNF-α) was investigated by western blot and quantitative real-time PCR (qRT-PCR). The fluorescent compound 4-(4-(dimethylamino)styryl) |
| Databáze: | OpenAIRE |
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