| Autor: |
De-jian, Xie, Ming-lei, Shi, Yan, Zhang, Tian-yi, Wang, Wen-long, Shen, Bing-yu, Ye, Ping, Li, Chao, He, Xiang-yuan, Zhang, Zhi-hu, Zhao |
| Rok vydání: |
2016 |
| Předmět: |
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| Zdroj: |
Yi chuan = Hereditas. 38(7) |
| ISSN: |
0253-9772 |
| Popis: |
The CCCTC-binding factor (CTCF) is the main insulator protein described in vertebrates. It plays fundamental roles during diverse cellular processes. CTCF gene knockout mice led to death during embryonic development. To further explore the functions of CTCF, we employed a CRISPR/Cas9-based genome engineering strategy to in-frame insert the mitosis-special degradation domain (MD) of cyclin B into the upstream open reading frame of CTCF gene. Fusion protein is designed to degrade during mitosis leaded by MD. As a control group, mutation of a single arginine (R42A) within the destruction box inactivates the MD leading to constitutive expression of MD |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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