| Autor: |
V A, Nikulina, E A, Kizim, Y S, Massino, O L, Segal, M B, Smirnova, V V, Avilov, D B, Saprygin, S P, Smotrov, G I, Kolyaskina, A D, Dmitriev |
| Rok vydání: |
1999 |
| Předmět: |
|
| Zdroj: |
Biochemistry. Biokhimiia. 64(10) |
| ISSN: |
0006-2979 |
| Popis: |
Using a panel of monoclonal antibodies against human myoglobin (Mb), we have shown that the sensitivity of antigen-capture ELISA can be significantly increased by simultaneous immobilization of two cooperating capture monoclonal antibodies on a solid phase. This method ("triple-site ELISA") uses three monoclonal antibodies to different epitopes of the same antigen (two capture/one tracer) unlike the traditional double-site assay using one capture and one tracer monoclonal antibody. We developed double- and triple-site ELISA for Mb by varying the capture and tracer monoclonal antibodies. Triple-site assays showed 4-6-fold increase in sensitivity compared to the double-site assays. A model for this effect is suggested; according to the model, in triple-site ELISA, high-affinity cyclic configurations can be formed by an antigen, two capture monoclonal antibodies, and the surface of the solid phase. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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