Hepatitis C virus core protein fused to hepatitis B virus core antigen for serological diagnosis of both hepatitis C and hepatitis B infections by ELISA
| Autor: | C L, Wu, T S, Leu, T T, Chang, A L, Shiau |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Male
Mice Inbred BALB C Time Factors Recombinant Fusion Proteins Viral Core Proteins Enzyme-Linked Immunosorbent Assay Hepatitis C Antibodies Hepatitis B Hepatitis B Core Antigens Hepatitis C Mice Animals Humans Electrophoresis Polyacrylamide Gel Female Serologic Tests Rabbits Hepatitis B Antibodies |
| Zdroj: | Journal of medical virology. 57(2) |
| ISSN: | 0146-6615 |
| Popis: | The sequence encoding the truncated core protein (amino acids 1-98) of hepatitis C virus (HCc) was expressed in E. coli for production of HCc(1-98), or fused with the truncated core antigen (HBcAg) and segments from the preS1 and preS2 regions from hepatitis B virus (HBV) for production of HBcPreS1PreS2HCc(1-98). The HCc(1-98) and HBcPreS1PreS2HCc(1-98) proteins reacted with sera from HCV-infected individuals by immunoblot analyses, while the latter protein also exhibited HBV core antigenicity. They induced antibodies against HBcAg and/or HCV core protein in rabbits and in mice. Moreover, HBcPreS1PreS2HCc(1-98) is more immunogenic than HCc(1-98) in terms of anti-HCc induction. An ELISA that employed recombinant HCV core antigens of either HCc(1-98) or HBcPreS1PreS2HCc(1-98) to detect anti-HCc and/or anti-HBc antibodies was developed. Evaluation of serum samples with different status of HBV and HCV infections suggested that HCc(1-98) might be suitable for the determination of antibodies against HCV core protein, while HBcPreS1PreS2HCc(1-98) might be of value to detect HCV and/or HBV infection in donated blood in HBV low-prevalence countries. |
| Databáze: | OpenAIRE |
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