Specific induction of Z-DNA conformation by a nuclear localization signal peptide of lupin glutaminyl tRNA synthetase

Autor:	A, Krzyzaniak, M, Siatecka, A, Szyk, P, Mucha, P, Rekowski, G, Kupryszewski, J, Barciszewski
Rok vydání:	2000
Předmět:	Amino Acyl-tRNA Synthetases Plants Medicinal DNA Plant Circular Dichroism Amino Acid Motifs Molecular Sequence Data Nuclear Localization Signals Nucleic Acid Conformation Fabaceae Amino Acid Sequence Peptide Fragments
Zdroj:	Molecular biology reports. 27(1)
ISSN:	0301-4851
Popis:	Recently we have sequenced cDNA of plant glutaminyl-tRNA synthetase (GlnRS) from Lupinus luteus. At the N terminal part the protein contains a lysine rich polypeptide (KPKKKKEK), which is identical to a nuclear localization signal (NLS). In this paper we showed that two synthetic peptides (20 and 8 amino acids long), which were derived from lupin GlnRS containing the NLS sequence interact with DNA, but one of them (8aa long) changing its conformation from the B to the Z form. This observation clearly suggests that the presence of the NLS polypeptide in a leader sequence of GlnRS is required not only for protein transport into nucleus but also for regulation of a gene expression. This is the first report suggesting a role of the NLS signal peptide in structural changes of DNA.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::b1a5d07d9013dc8a7fbacdb04d3b1baa https://pubmed.ncbi.nlm.nih.gov/10939526 Zobrazit plný text záznamu Full text from SpringerLink