Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps

Autor: Phillipa C, White, Ilaria J, Chicca, Martin R, Ling, Helen J, Wright, Paul R, Cooper, Mike R, Milward, Iain L C, Chapple
Rok vydání: 2016
Předmět:
Zdroj: Methods in molecular biology (Clifton, N.J.). 1537
ISSN: 1940-6029
Popis: Following the discovery of neutrophil extracellular traps (NETs) in 2004 by Brinkmann and colleagues, there has been extensive research into the role of NETs in a number of inflammatory diseases, including periodontitis. This chapter describes the current methods for the isolation of peripheral blood neutrophils for subsequent NET experiments, including approaches to quantify and visualize NET production, the ability of NETs to entrap and kill bacteria, and the removal of NETs by nuclease-containing plasma.
Databáze: OpenAIRE