Fasciola hepatica cathepsin L cysteine proteinase suppresses Bordetella pertussis-specific interferon-gamma production in vivo

Autor:	S M, O'Neill, K H, Mills, J P, Dalton
Rok vydání:	2001
Předmět:	Immunosuppression Therapy Mice Knockout Mice Inbred BALB C Cathepsin L Fasciola hepatica Ketones Cathepsins Bordetella pertussis Cysteine Endopeptidases Interferon-gamma Mice Diazomethane Bacterial Vaccines Animals Female Immunization Interleukin-4 Enzyme Inhibitors
Zdroj:	Parasite immunology. 23(10)
ISSN:	0141-9838
Popis:	We have previously demonstrated that Fasciola hepatica infection significantly reduced Bordetella pertussis-specific interferon (IFN)-gamma production in mice coinfected with B. pertussis or immunized with a pertussis whole cell vaccine (Pw). In the present study, we have identified parasite molecules capable of mimicking this suppressive effect of F. hepatica. Parenteral injection of mice with culture medium in which adult F. hepatica were maintained (excretory/secretory, ES, products) suppressed B. pertussis-specific IFN-gamma production in mice immunized with Pw. The suppressive effect of ES was abrogated by coinjecting ES with the cysteine proteinase inhibitor, Z-Phe-Ala-diazomethylketone. Furthermore, purified cathepsin L proteinase (FheCL), a major component of ES products, was capable of suppressing IFN-gamma production. The suppressive effect of FheCL was attenuated in interleukin (IL)-4 defective (IL-4-/-) mice. Therefore, FheCL released by F. hepatica is involved in the suppression of Th1 immune responses and this suppression may be dependent upon IL-4.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::b182c6786a7387e357859941bc333b4b https://pubmed.ncbi.nlm.nih.gov/11696165 Zobrazit plný text záznamu Plný text