Dietary
| Autor: | Kang, Xie, Yangli, Liu, Xixia, Li, Hong, Zhang, Shuyan, Zhang, Ho Yi, Mak, Pingsheng, Liu |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Male
Lipogenesis Stenotrophomonas maltophilia Lipid Droplets Endoplasmic Reticulum Gastrointestinal Microbiome Stenotrophomonas maltophilia/obesity/dhs-3/mdt-28/lipid droplet Animals Female Caenorhabditis elegans Caenorhabditis elegans Proteins Stearoyl-CoA Desaturase Transcription Factors Research Article Research Paper |
| Zdroj: | Gut Microbes article-version (VoR) Version of Record |
| ISSN: | 1949-0984 |
| Popis: | Dietary and symbiotic bacteria can exert powerful influence on metazoan lipid metabolism. Recent studies have emerged that microbiota have a role in animal obesity and related health disorders, but the mechanisms by which bacteria influence lipid storage in their host are unknown. To reduce the complexity of the relationship between gut microbiota and the host, Caenorhabditis elegans (C. elegans) has been chosen as a model organism to study interspecies interaction. Here, we demonstrate that feeding C. elegans with an opportunistic pathogenic bacterium Stenotrophomonas maltophilia (S. maltophilia) retards growth and promotes excessive neutral lipid storage. Gene expression analysis reveals that dietary S. maltophilia induces a lipogenic transcriptional response that includes the SREBP ortholog SBP-1, and fatty acid desaturases FAT-6 and FAT-7. Live imaging and ultrastructural analysis suggest that excess neutral lipid is stored in greatly expanded lipid droplets (LDs), as a result of enhanced endoplasmic reticulum (ER)-LD interaction. We also report that loss of function mutations in dpy-9 in C. elegans confers resistance to S. maltophilia. Dietary S. maltophilia induces supersized LDs by enhancing lipogenesis and ER-LD contacts in C. elegans. This work delineates a new model for understanding microbial regulation of metazoan physiology. |
| Databáze: | OpenAIRE |
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