| Autor: |
Camila, Arrivabene Neves, Lucilene, Dos Santos Silva, Camila Ernanda Sousa, de Carvalho, Marina, Silva Carvalho, José Lindenberg Rocha, Sarmento, Tânia, Vasconcelos Cavalcante, Mônica, Arrivabene, Tábatta, Arrivabene Neves, Maria Éllida, de Sousa Bezerra, Antônio Luiz Gomes, Júnior, Cleidson Manoel Gomes, da Silva, Maria Acelina Martins, de Carvalho |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Zygote (Cambridge, England). 28(1) |
| ISSN: |
1469-8730 |
| Popis: |
This study aims to develop an in vitro co-culture system of in situ goat preantral follicles with bone marrow-derived mesenchymal stem cells (BM-MSC), evaluating the influence of these cells on follicular growth, rate of activation and morphologically normal follicles. Fragments of ovarian cortex were cultured for 1 or 7 days in the presence of BM-MSC (BM-MSC+) and absence of BM-MSC (BM-MSC-). Histological sections of the fragments were analysed and data were obtained regarding morphological classification, survival rate of morphologically normal follicles and rate of follicular activation. Culture medium on days 1 and 7 was also sampled for nitrite concentration and reduced glutathione activity. There was a reduction (P0.05) in the percentage of morphologically normal follicles in the BM-MSC+ compared with the fresh control only on the seventh day of culture. When comparing treatments, on the seventh day of culture, a higher rate of morphologically normal preantral follicles was observed in BM-MSC+ (P0.05). In both treatments, primordial and developing follicle rates were similar to the fresh control (P0.05). When comparing treatments with each other, as well as with the fresh control, no differences were observed in follicular diameter (P0.05) or nitrite concentration (P0.05). The concentration of reduced glutathione was lower on the seventh day of co-culture in both treatments (P0.05). In conclusion, co-culture had no influence on follicular or oocyte development. However, it was critical to maintain the survival of preantral follicles during 7 days of culture. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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