Yeast Ty retrotransposons assemble into virus-like particles whose T-numbers depend on the C-terminal length of the capsid protein

Autor:	H A, AL-Khayat, D, Bhella, J M, Kenney, J F, Roth, A J, Kingsman, E, Martin-Rendon, H R, Saibil
Rok vydání:	1999
Předmět:	Fungal Proteins Capsid Retroelements Protein Conformation Cryoelectron Microscopy Image Processing Computer-Assisted Saccharomyces cerevisiae Particle Size
Zdroj:	Journal of molecular biology. 292(1)
ISSN:	0022-2836
Popis:	The virus-like particles (VLPs) produced by the yeast Ty retrotransposons are structurally and functionally related to retroviral cores. Using cryo-electron microscopy (cryo-EM) and three-dimensional (3D) reconstruction, we have examined the structures of VLPs assembled from full-length and truncated forms of the capsid structural protein. The VLPs are highly polydisperse in their radius distribution. We have found that the length of the C-terminal region of the capsid structural protein dictates the T -number, and thus the size, of the assembled particles. Each construct studied appears to assemble into at least two or three size classes, with shorter C termini giving rise to smaller particles. This assembly property provides a model for understanding the variable assembly of retroviral core proteins. The particles are assembled from trimer-clustered units and there are holes in the capsid shells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::b0bb290fdd086b444cdf2688f2696554 https://pubmed.ncbi.nlm.nih.gov/10493857 Zobrazit plný text záznamu Full Text from ScienceDirect