Nondestructive, indirect assessment of the biomechanical properties of the rat intervertebral disc using contrast-enhanced μCT

Autor:	Michael D, Newton, Samantha E, Hartner, Karissa, Gawronski, Erik J, Davenport, Shannon C, Timmons, Kevin C, Baker, Tristan, Maerz
Rok vydání:	2017
Předmět:	Cartilage Articular Lumbar Vertebrae Contrast Media Reproducibility of Results Intervertebral Disc Degeneration X-Ray Microtomography Biomechanical Phenomena Rats Rats Inbred Lew Ioxaglic Acid Animals Female Stress Mechanical Intervertebral Disc Intervertebral Disc Displacement Glycosaminoglycans
Zdroj:	Journal of orthopaedic research : official publication of the Orthopaedic Research Society. 36(7)
ISSN:	1554-527X
Popis:	Mechanical characterization of the intervertebral disc involves labor-intensive and destructive experimental methodology. Contrast-enhanced micro-computed tomography is a nondestructive imaging modality for high-resolution visualization and glycosaminoglycan quantification of cartilaginous tissues. The purpose of this study was to determine whether anionic and cationic contrast-enhanced micro-computed tomography of the intervertebral disc can be used to indirectly assess disc mechanical properties in an ex vivo model of disc degeneration. L3/L4 motion segments were dissected from female Lewis rats. To deplete glycosaminoglycan, samples were treated with 0 U/ml (Control) or 5 U/ml papain. Contrast-enhanced micro-computed tomography was performed following incubation in 40% Hexabrix (anionic) or 30 mg I/ml CA
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::b0b90bb6d38eacfb0b221c85d18c0b43 https://pubmed.ncbi.nlm.nih.gov/29314237 Zobrazit plný text záznamu Plný text