Sequence and functional analysis of the 5'-flanking region of the mouse growth inhibitory factor gene
| Autor: | M, Watabe, S, Gross, C, Lawyer, G J, Brewer, T, Mashimo, K, Watabe |
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| Rok vydání: | 1997 |
| Předmět: |
Neurons
Genomic Library Base Sequence Transcription Genetic Recombinant Fusion Proteins Molecular Sequence Data Nerve Tissue Proteins 3T3 Cells Glioma Regulatory Sequences Nucleic Acid Transfection Polymerase Chain Reaction Growth Inhibitors Metallothionein 3 Rats Mice Tumor Cells Cultured Animals Cloning Molecular Neuroglia Cells Cultured |
| Zdroj: | Cellular and molecular neurobiology. 17(2) |
| ISSN: | 0272-4340 |
| Popis: | 1. The growth inhibitory factor (GIF) is a 68-amino acid protein which is capable of inhibiting the growth of neuronal cells in vitro. 2. We have cloned and sequenced the 5'-flanking region of the mouse GIF gene, which spans from the transcriptional initiation site to the -1854 nucleotide. 3. This region contains sequences homologous to hgcs, SPE, and the JCV silencer domain that functions in a glial cell specific manner. This region also contains two metal responding elements and putative binding sites for AP-1, AP-2, Sp-1, SP-2, NF-1, and CREB. 4. An analysis of the reporter plasmids containing the various regions of the 5'-flanking sequence revealed that the region indeed functioned in a tissue-specific manner in glial cells and that the region between -328 and 175 is responsible for suppression, while the region between -175 and -49 is involved in the activation of gene expression. |
| Databáze: | OpenAIRE |
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