Molecular cloning of rodent p72Syk. Evidence of alternative mRNA splicing
| Autor: | R B, Rowley, J B, Bolen, J, Fargnoli |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Enzyme Precursors
DNA Complementary Genome ZAP-70 Protein-Tyrosine Kinase Base Sequence Sequence Homology Amino Acid Molecular Sequence Data Intracellular Signaling Peptides and Proteins Exons Sequence Analysis DNA Protein-Tyrosine Kinases Blotting Northern Introns Rats Alternative Splicing Species Specificity Tumor Cells Cultured Animals Syk Kinase Amino Acid Sequence Cloning Molecular |
| Zdroj: | The Journal of biological chemistry. 270(21) |
| ISSN: | 0021-9258 |
| Popis: | Northern blot analysis of polyadenylated RNA prepared from RBL-2H3 cells revealed the presence of three distinct mRNAs encoding p72Syk, a protein-tyrosine kinase previously shown to be associated with the high affinity IgE receptor present on the surface of these cells (Hutchcroft, J. E., Geahlen, R. L., Deanin, G. G., and Oliver, J. M. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 9107-9111). Here we report the full-length nucleotide sequence of two of these messages, as well as the complete predicted amino acid sequence of the rodent p72Syk protein-tyrosine kinase. In addition, we report evidence indicating alternative splicing of p72Syk mRNAs within RBL-2H3 cells. This splicing event results in the expression of two distinct protein isoforms that differ with respect to the presence of a 23-amino acid insert located within the region of the protein that separates the two SH2 domains from the catalytic domain. Both mRNAs arising from this splicing event appear to encode functional protein-tyrosine kinases, as expression of the corresponding cDNAs in COS cells results in the production of proteins of the expected sizes that possess intrinsic tyrosine specific kinase activity. |
| Databáze: | OpenAIRE |
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