Simvastatin attenuates intestinal ischemia/reperfusion induced injury in rat
| Autor: | B, Hajipour, M H, Somi, F, Saberifar, M R, Hemmati, N A, Asl, A, Moein, A M, Vatankhah, A R, Nourazar, M R, Nasirizade |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Male
Simvastatin Superoxide Dismutase Tumor Necrosis Factor-alpha Down-Regulation Free Radical Scavengers Glutathione Antioxidants Rats Disease Models Animal Intestinal Diseases Oxidative Stress Treatment Outcome Ischemia Regional Blood Flow Malondialdehyde Reperfusion Injury Animals Hydroxymethylglutaryl-CoA Reductase Inhibitors Rats Wistar |
| Zdroj: | Folia morphologica. 68(3) |
| ISSN: | 0015-5659 |
| Popis: | Ischaemia/reperfusion (I/R) injury is commonly seen in the field of intestine surgical interventions, shock, trauma, and many other clinical conditions. Simvastatin is known to have antioxidant and anti-inflammatory properties. This study investigated the effect of simvastatin administration in a warm intestinal I/R model on TNF-alpha, antioxidant enzymes and intestinal tissue morphology. Thirty-six male wistar rats underwent laparotomy under general anaesthesia. Simvastatin was administered from four days before ischaemia induction. The rats were divided in to three groups (n = 12): the sham group, the I/R group, and the I/R + simvastatin group. Intestinal ischaemia was induced by superior mesenteric artery ligation with microvascular clamps for 60 minutes, and after ischaemia, blood perfusion was released into the tissue and a reperfusion phase was started, which lasted for 3 hours. After 3 hours, the animals were sacrificed and serum and tissue obtained for biochemical and histological study. In the simvastatin treated group, intestinal tissue injury, TNF-alpha level, and tissue malondealdehyde levels were significantly lower than in the I/R group (p0.05). Glutathion peroxidase and superoxide dismutase levels were significantly higher in the simvastatin treated group than in the I/R group (p0.05). Simvastatin pretreatment reduced intestinal I/R injury and was associated with down- -regulation of serum TNF-alpha and tissue malondealdehyde level, and simvastatin administration maintained cellular antioxidant enzyme contents compared to the I/R group after 3 hours reperfusion time. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|