Comparison of three molecular methods for typing Aeromonas popoffii isolates

Autor:	Lara, Soler, Maria Jose, Figueras, Matilde R, Chacón, Josep, Guarro, Antonio J, Martinez-Murcia
Rok vydání:	2003
Předmět:	DNA Bacterial RNA Ribosomal 23S Belgium Scotland RNA Ribosomal 16S DNA Ribosomal Spacer Fresh Water Seawater Aeromonas Polymerase Chain Reaction Polymorphism Restriction Fragment Length Switzerland Bacterial Typing Techniques
Zdroj:	Antonie van Leeuwenhoek. 83(4)
ISSN:	0003-6072
Popis:	Three typing methods, restriction fragment length polymorphism (RFLP) of the 16S-23S intergenic spacer region (ISR), PCR amplification of the enterobacterial repetitive intergenic consensus (ERIC) and of the repetitive extragenic palindromic units (REP), were evaluated for typing 26 isolates of Aeromonas popoffii from different geographical origins. When the methods were independently studied, ERIC showed the highest discriminatory power. When the methods were combined, the best combination of two methods was ERIC with REP since strains showed a tendency to cluster according to their geographical origin. However, this tendency was reinforced with the addition of ISR-RFLP.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::b00fac21eb287d073380d408c28d814c https://pubmed.ncbi.nlm.nih.gov/12777070 Zobrazit plný text záznamu Full text from SpringerLink