Protein encapsulation in biodegradable polymeric nanoparticles: morphology, fluorescence behaviour and stem cell uptake
| Autor: | Nicoletta, Rescignano, Luigi, Tarpani, Roberto, Tiribuzi, Simona, Montesano, Sabata, Martino, Loredana, Latterini, Josè Maria, Kenny, Ilaria, Armentano |
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| Rok vydání: | 2013 |
| Předmět: |
Calorimetry
Differential Scanning Temperature Biocompatible Materials Bone Marrow Cells Mesenchymal Stem Cells Serum Albumin Bovine Biodegradation Environmental Immobilized Proteins Spectrometry Fluorescence Polylactic Acid-Polyglycolic Acid Copolymer Thermogravimetry Animals Humans Nanoparticles Cattle Lactic Acid Cells Cultured Fluorescein-5-isothiocyanate Polyglycolic Acid |
| Zdroj: | Macromolecular bioscience. 13(9) |
| ISSN: | 1616-5195 |
| Popis: | The synthesis and characterization of new biodegradable polymeric NPs loaded with bovine serum albumin marked with fluorescein isothiocyanate (FITC-BSA) is reported. The protein is encapsulated in poly(D,L-lactide-co-glycolide) (PLGA) NPs by the double emulsion method with subsequent solvent evaporation. The NPs display a spherical shape with a narrow size distribution and no aggregation is observed after drying. Steady-state and time-resolved fluorescence measurements appear to be a sensitive method to investigate the protein environment on the nanometer-scale. Finally, FITC-BSA-loaded NPs are rapidly internalized in stem cells. Interestingly, 25% cells were slightly positive after 28 days. |
| Databáze: | OpenAIRE |
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