| Autor: |
Fan-Ping, Wang, Cai-Juan, Qiao, Yan-Wei, Sun, Xiang-Yang, Li, Xiao-Yu, Huang, Wen-Rui, Zhang, Xia, Wang, Ming-Yong, Wang |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Zhongguo shi yan xue ye xue za zhi. 29(4) |
| ISSN: |
1009-2137 |
| Popis: |
To investigate the effect of sulforaphane (SFN) on GKG1a and KG1cells were treated by different concentrations of SFN for 48 h. Flow cytometry (FCM) was used to analyze the phase distribution of cell cycle. High-throughput sequencing was used to detect the effect of SFN on the expression of cell cycle related genes in KG1a cells. The mRNA expression of P53, P21, CDC2 and CyclinB1 were detected by qPCR. The protein expression of P53, CDC2, P-CDC2 and CyclinB1 were detected by Western blot.Cells in the GSFN induces leukemia cells to block in G莱菔硫烷诱导急性髓系白血病KG1a和KG1细胞G研究莱菔硫烷（sulforaphane, SFN）对急性髓系白血病细胞G不同浓度的SFN作用KG1a和KG1细胞48 h后，流式细胞术检测细胞周期的变化, 利用高通量转录组测序技术检测SFN对KG1a细胞周期相关基因表达情况的影响；采用实时荧光定量PCR（qPCR）检测P53、P21、CDC2、CyclinB1的mRNA表达。采用Western blot检测 P53、CDC2、P-CDC2和CyclinB1的蛋白表达.经SFN作用48 h后，KG1a和KG1细胞的GSFN可能通过激活P53信号通路，进一步抑制CDC2表达和CDC2/CyclinB1复合物的活性，诱导急性髓系白血病KG1a和KG1细胞阻滞在G |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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