| Autor: |
O V, Solov'eva, D L, Kakuev, N V, Gibanova, V M, Lipkin |
| Rok vydání: |
2000 |
| Předmět: |
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| Zdroj: |
Bioorganicheskaia khimiia. 26(6) |
| ISSN: |
0132-3423 |
| Popis: |
The C type natriuretic peptide (CNP) is a peptide hormone stimulating vasorelaxation and inhibiting cell proliferation. CNP activates the type B natriuretic peptide receptor (NPR-B), known as the guanylate cyclase B membrane enzyme, which results in the cGMP release. To study functional properties of NPR-B, its gene fragments were expressed in methylotrophic yeasts Pichia pastoris. Conditions were found providing for secretion of functionally active recombinant proteins NPR-Bs and NPR-B1 into the cultural medium in a yield of 25 mg/ml culture. Their specific activity was 0.97 and 0.93 mumol cGMP min-1 mg-1 protein, respectively. It was shown that NPR-B belongs to the family of Ser/Thr protein kinases and can be autophosphorylated at the serine residues. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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