HIV-1 env DNA vaccine administered to rhesus monkeys elicits MHC class II-restricted CD4+ T helper cells that secrete IFN-gamma and TNF-alpha
| Autor: | C, Lekutis, J W, Shiver, M A, Liu, N L, Letvin |
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| Rok vydání: | 1997 |
| Předmět: |
AIDS Vaccines
CD4-Positive T-Lymphocytes Tumor Necrosis Factor-alpha Molecular Sequence Data Histocompatibility Antigens Class II Gene Products env T-Lymphocytes Helper-Inducer HIV Envelope Protein gp120 Lymphocyte Activation Macaca mulatta Interferon-gamma DNA Viral HIV-1 Animals Amino Acid Sequence Peptides Epitope Mapping |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 158(9) |
| ISSN: | 0022-1767 |
| Popis: | The Th cell response elicited by an HIV-1 env plasmid DNA vaccine was assessed in rhesus monkeys by isolation of gp120-specific, MHC class II-restricted CD4+ T cell lines from PBL of vaccinated animals. The Env-specific CD4+ T cell lines recognized epitopes located in the second hypervariable region and in the carboxyl terminus of HIV-1 gp120. These cell lines proliferated in response to APC in the presence of recombinant gp120, as well as to APC expressing virally encoded Env. All of the CD4+ T cell lines responded to Env peptide by secreting IFN-gamma and TNF-alpha without appreciable IL-4 production. Recombinant gp120 stimulation of PBL from these vaccinated monkeys elicited the secretion of a similar profile of cytokines. Demonstration of a nucleotide vaccine eliciting a Th1-like immune response is consistent with the well documented ability of naked DNA vaccines to induce durable CD8+ CTL responses. |
| Databáze: | OpenAIRE |
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