| Autor: |
Hazal Banu, Olgun, Hale M, Tasyurek, Ahter D, Sanlioglu, Salih, Sanlioglu |
| Rok vydání: |
2018 |
| Předmět: |
|
| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 1879 |
| ISSN: |
1940-6029 |
| Popis: |
Lentiviral vectors (LVs) have been increasingly used in clinical gene therapy applications particularly due to their efficient gene transfer ability, lack of interference from preexisting viral immunity, and long-term gene expression they provide. Purity of LVs is essential in in vivo applications, for a high therapeutic benefit with minimum toxicity. Accordingly, laboratory scale production of LVs frequently involves transient cotransfection of 293T cells with packaging and transfer plasmids in the presence of CaPO |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
