Genotyping of clinical Rhodotorula mucilaginosa isolates by pulsed field gel electrophoresis

Autor:	M A, Saracli, K, Sener, A, Gonlum, S T, Yildiran, B L, Wickes
Rok vydání:	2003
Předmět:	Male Molecular Epidemiology Genotype Turkey Prostate Rhodotorula DNA Fingerprinting Electrophoresis Gel Pulsed-Field Random Amplified Polymorphic DNA Technique Mycoses Karyotyping Humans Mycological Typing Techniques Fungemia
Zdroj:	Mycoses. 46(11-12)
ISSN:	0933-7407
Popis:	Identification and typing of fungal isolates is a prerequisite for control and prevention of nosocomial infections. As the discriminatory power of phenotypic methods is not sufficient for epidemiological purposes, genotyping methods such as DNA fingerprinting, random amplification of polymorphic DNA (RAPD) analysis, or pulsed field gel electrophoresis (PFGE) are preferred. To our knowledge, this study is the first application of PFGE for typing Rhodotorula mucilaginosa strains. The PFGE patterns of six clinical isolates produced two different karyotypes, which were confirmed by RAPD analysis. Five strains isolated from bloodstream infections from three different institutions showed the same karyotype and RAPD patterns, while the urological specimen differed slightly.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::af2831384e6286f2e74a345d70f517be https://pubmed.ncbi.nlm.nih.gov/14641622 Zobrazit plný text záznamu Plný text