| Autor: |
E S, Zubkova, K V, Dergilev, I B, Beloglazova, Yu D, Molokotina, M A, Boldyreva, Z I, Tsokolaeva, I S, Stafeev, M Yu, Menshikov, Ye V, Parfyonova |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Bulletin of experimental biology and medicine. 171(4) |
| ISSN: |
1573-8221 |
| Popis: |
Murine peritoneal macrophages isolated from the lavage fluid after administration of thioglycolate and concanavalin A are presented by two populations of cells of different diameters. Polarization of macrophages into a proinflammatory (M1) phenotype is accompanied by an increase in number of small cells. Macrophages obtained after administration of thioglycolate demonstrate higher tendency to anti-inflammatory (M2) phenotype, while macrophages isolated after administration of concanavalin A are committed in the proinflammatory direction. Lactate level is increased in M1 macrophages in comparison with M2 cells, which indicates predominance of glycolytic metabolism. Macrophages obtained after administration of concanavalin A have reduced mitochondrial potential, which reflects a tendency to apoptosis. Autophagy activation and inhibition neutralize the differences in pro- and anti-inflammatory properties of polarized macrophages obtained after thioglycolate administration, but have less pronounced effect on macrophages obtained after administration concanavalin A. Autophagy inhibitor increases mitochondrial potential in non-polarized macrophages obtained after administration of concanavalin A. These results demonstrate divergent properties of macrophages obtained after administration of glycolate and concanavalin A due to the difference in the mechanisms of experimental peritonitis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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