| Autor: |
A K, Nakazone, J R, Rogero, J M, Gonçalves |
| Rok vydání: |
1984 |
| Předmět: |
|
| Zdroj: |
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas. 17(2) |
| ISSN: |
0100-879X |
| Popis: |
p6ospholipase A and crotapotin were purified by gel filtration and ion-exchange chromatography from Crotalus durissus terrificus venom. The minimal molecular weights obtained by amino acid analysis were 13,400 daltons for phospholipase A and 8,300 daltons for crotapotin. Phospholipase A and crotapotin form a 1:1 molar complex that was stable during gel filtration. The LD50 of phospholipase A was increased about 10-fold when in a 1:1 complex with crotapotin. Crotapotin, phospholipase A and crotoxin (crotapotin + phospholipase A) gave precipitin reactions when tested with specific rabbit antisera. Immunological studies of these proteins using crotalus antiserum confirmed 1:1 molar ratio of the complex. Crotoxin antiserum also reacts with either crotapotin or phospholipase A separately. Similar antigenic determinants were observed in the subunits and it seems that some antigenic determinants are masked in the phospholipase A-crotapotin complex. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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