Multicenter evaluation of the Candida albicans/Candida glabrata peptide nucleic acid fluorescent in situ hybridization method for simultaneous dual-color identification of C. albicans and C. glabrata directly from blood culture bottles

Autor: Janeen R, Shepard, Rachel M, Addison, Barbara D, Alexander, Phyllis, Della-Latta, Michael, Gherna, Gerhard, Haase, Gerri, Hall, Jennifer K, Johnson, William G, Merz, Heidrun, Peltroche-Llacsahuanga, Henrik, Stender, Richard A, Venezia, Deborah, Wilson, Gary W, Procop, Fann, Wu, Mark J, Fiandaca
Rok vydání: 2007
Předmět:
Zdroj: Journal of clinical microbiology. 46(1)
ISSN: 1098-660X
Popis: We evaluated the performance of the Candida albicans/Candida glabrata peptide nucleic acid fluorescent in situ hybridization (PNA FISH) method, a rapid two-color assay for detection of C. albicans and C. glabrata, in a multicenter study. The assay is designed for use directly from positive blood culture bottles in a FISH format. Intact, fixed cells are labeled fluorescent green (C. albicans) or fluorescent red (C. glabrata) by rRNA hybridization of fluorophore-labeled PNA probes. Results are available
Databáze: OpenAIRE