| Autor: |
Marie-Cecile, van de Lavoir, Christine, Mather-Love |
| Rok vydání: |
2006 |
| Předmět: |
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| Zdroj: |
Methods in enzymology. 418 |
| ISSN: |
0076-6879 |
| Popis: |
Blastodermal cells derived from the area pellucida of a stage X (EGK) embryo have the potential to contribute to the somatic tissues and the germ line when reintroduced into a stage X (EGK) recipient embryo. This chapter describes a method to culture chicken embryonic stem (cES) cells derived from blastodermal cells. Within the first week of culture, the cells change their morphology; they become smaller with a large nucleus and a prominent nucleolus. The cES cells remain chromosomally normal and can be cultured for extended periods. They can be modified genetically using standard electroporation procedures and, after injection into a recipient embryo, can contribute to all somatic tissues. Using a surrogate shell culture system, the injected embryos can be manipulated and visualized easily throughout incubation. We have generated high-grade chimeras by compromising the recipient embryos and maintaining the ES cells in stage X (EGK) recipients for a few days at 15 degrees before incubating them at 37.5 degrees. The cES system provides a novel experimental paradigm for the investigation of developmental and physiological mechanisms in the chicken. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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