A Fast and Easy Technique for the Isolation of Aleurone Layers 1
| Autor: | Murthy, Pushpalatha P. N. |
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| Jazyk: | angličtina |
| Rok vydání: | 1989 |
| Předmět: | |
| Popis: | Although the aleurone layer of ceral grain seeds has many advantages for the study of gibberellin action, it has the disadvantage that the hand-isolation of the aleurone layers is time-consuming. To overcome this disadvantage, a commercially available pasta machine was modified and used to remove aleurone layers from imbibed barley (Hordeum vulgare) seeds. This equipment allows isolation of a thousand layers in 5 minutes compared to the 3 to 4 hours required to hand-isolate them. The machine-made aleurone layers are gibberellic-acid responsive and the response is similar both qualitatively and quantitatively to that of hand-isolated layers. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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