All-trans retinoic acid regulates the expression of the extracellular matrix protein fibulin-1 in the guinea pig sclera and human scleral fibroblasts
| Autor: | Chuanxu, Li, Sally A, McFadden, Ian, Morgan, Dongmei, Cui, Jianmin, Hu, Wenjuan, Wan, Junwen, Zeng |
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| Rok vydání: | 2009 |
| Předmět: |
Extracellular Matrix Proteins
genetic structures Adolescent Cell Survival Calcium-Binding Proteins Guinea Pigs Cell Count Tretinoin Fibroblasts Immunohistochemistry eye diseases Coculture Techniques Young Adult Gene Expression Regulation Animals Humans sense organs Aggrecans RNA Messenger Child Cells Cultured Sclera Research Article |
| Zdroj: | Molecular Vision |
| ISSN: | 1090-0535 |
| Popis: | Purpose Fibulin-1 (FBLN1) mRNA is expressed in human sclera and is an important adhesion modulatory protein that can affect cell–matrix interactions and tissue remodeling. Scleral remodeling is influenced by all-trans retinoic acid (RA). Our purpose was to confirm the presence of fibulin-1 protein in guinea pig sclera and investigate the effect of RA on the expression of fibulin-1 in guinea pig sclera in vivo and in cultured human scleral fibroblasts (HSFs). Methods Confocal fluorescence microscopy was used to study fibulin-1 and aggrecan expression and localization in sclera from control guinea pigs and in animals given RA by daily gavage from 4 to 8 days of age. The effects of RA (from 10−9 to 10−5 M) on fibulin-1 expression in HSFs were observed by immunohistochemistry and assayed by real-time PCR and western blot analysis. Results Fibulin-1 protein expression was detected by confocal fluorescence microscopy in guinea pig sclera and in cultured HSFs. Upregulation of fibulin-1 in scleral tissue was observed after feeding with RA. In vitro, the level of Fbln1 mRNA was increased after treatment of HSFs with RA (at concentrations of 10−8 to 10−6 M; p |
| Databáze: | OpenAIRE |
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