RNA Synthesis by DNA Methyltransferase 1 siRNA Transfection with Cationic Liposomes into Osteoblastic Progenitor Cells Based on SiO² Nanomagnetic Beads for Proliferation and Differentiation

Autor:	Qingzhen, Chen, Tao, Jiang, Qinshen, Wang, Yongqing, Huang, Min, Shao
Rok vydání:	2020
Předmět:	Osteoblasts Stem Cells Cell Differentiation 3T3 Cells DNA Methyltransferases Silicon Dioxide Transfection Mice Osteogenesis Liposomes Animals Nanoparticles RNA Small Interfering Cell Proliferation
Zdroj:	Journal of nanoscience and nanotechnology. 20(10)
ISSN:	1533-4899
Popis:	DNA methylation regulated gene expression is important for osteoblast proliferation and differentiation during bone remodeling and its deregulation leads to the development of osteoporosis. DNA methyltransferase 1 (DNMT1) is an important regulator of DNA methylation. To explore the effect and mechanism of differential expression of DNMT1 in osteoblast precursor cells, DNMT1 siRNAs were designed and synthesized to interfere with DNMT1 expression in the osteoblast precursor cells, MC3T3E1 (Clone 24; MC3T3E1-24). The expression of the target gene, DNMT1, and osteogenic differentiation indicators osteoprotegerin (OPG) and receptor activator of nuclear factor
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::adbf701f7119b23ef1fe8c911f524eb0 https://pubmed.ncbi.nlm.nih.gov/32384955 Zobrazit plný text záznamu