| Autor: |
C, Lacroix, F, Cerutti, J, Nouveau, S, Menager, O, Lafont |
| Jazyk: |
francouzština |
| Rok vydání: |
1987 |
| Předmět: |
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| Zdroj: |
Journal of chromatography. 415(1) |
| Popis: |
The method presented here is based on the ability of ethambutol to give chelates with divalent cationic metals. With copper salts, the chelate presents a characteristic ultraviolet absorbance at 270 nm, making it possible to titrate ethambutol extracted from plasma with chloroform in alkaline medium. The column which was used contained silica (LiChrosorb Si 60, 5 microns) and the mobile phase was a mixture of equal parts of water and acetonitrile, containing copper sulphate and ammonia. The internal standard was a lower homologue of ethambutol with the same chelating ability. The detection limit was 0.15 mg/l. No interference with other antitubercular agents was observed. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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