Phytochemical Analysis of a Cytotoxic Fraction of Quassia silvestris using LC-HR-ESI-MS

Autor: Michel Feussi, Tala, Ferdinand Mouafo, Talontsi, Guang-Zhi, Zeng, Hippolyte Kamdem, Wabo, Michael, Spiteller, Ning-Hua, Tan, Pierre, Tane
Rok vydání: 2016
Předmět:
Zdroj: Phytochemical analysis : PCA. 28(3)
ISSN: 1099-1565
Popis: The genus Quassia is a promising source of secondary metabolites with biological potential including antimalarial and cytotoxic activities. Limited data are available on the phytochemistry and pharmacology of Quassia silvestris CheekJongkind, a Cameroonian medicinal plant used to treat various ailments.To carry out the bioassay-guided fractionation and LC-HR-ESI-MS analyses of the leaves extract from Q. silvestris; to purify the active fractions and isolate the major compounds using different chromatographic and spectroscopic methods. The obtained compounds will be evaluated for their biological activity.Following the cytotoxic screening and LC-HR-ESI-MS profiling of fractions obtained from partition of the methanolic extract of Q. silvestris leaves, the CHSixteen squalene-derived metabolites were identified with oxasqualenoid derivatives being the most predominant. Among the isolates, structure elucidation of two new oxasqualenoids quassiols E (1) and F (2), were achieved by NMR (one-dimensional (1D) and two-dimensional (2D)) and MS methods. The newly characterised compounds 1 and 2, together with the known tetraol (3) and 3-oxo-oleanoic acid (16) displayed moderate cytotoxicity.The identification and structural characterisation of highly oxidised squalene derived metabolites from this plant may provide important insight data for further pharmacological investigations. The LC-HR-ESI-MS
Databáze: OpenAIRE