Derivatization and template-guided ligation of oligodeoxyribonucleotides using cyanogen bromide and N-substituted morpholines

Autor:	Z A, Shabarova, O A, Fedorova, N G, Dolinnaya, M B, Gottikh
Rok vydání:	2001
Předmět:	Evolution Molecular Oligodeoxyribonucleotides Morpholines Oligonucleotides Cyanogen Bromide DNA Templates Genetic Buffers
Zdroj:	Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life. 27(5-6)
ISSN:	0169-6149
Popis:	Cyanogen bromide has been found to induce the template-guided condensation of oligonucleotides only in the presence of N-substituted morpholines. Based on 31P, 1H and 13C NMR spectroscopy data, the mechanism of the phosphomonoester group activation by cyanogen bromide in N-substituted morpholine buffers is suggested. It has also been shown that BrCN can be used for the synthesis of oligonucleotide derivatives in aqueous solution.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::acc8e5ccd9bd566563b2746539c3094f https://pubmed.ncbi.nlm.nih.gov/11536842 Zobrazit plný text záznamu Full text from SpringerLink