Bacterial expression of antibody fragments that block human rhinovirus infection of cultured cells
| Autor: | J H, Condra, V V, Sardana, J E, Tomassini, A J, Schlabach, M E, Davies, D W, Lineberger, D J, Graham, L, Gotlib, R J, Colonno |
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| Rok vydání: | 1990 |
| Předmět: |
Genes
Immunoglobulin Rhinovirus Protein Conformation Molecular Sequence Data Antibodies Monoclonal Gene Expression Cell Transformation Viral Recombinant Proteins Immunoglobulin Fab Fragments Escherichia coli Humans Receptors Virus Immunoglobulin Light Chains Amino Acid Sequence Cloning Molecular Immunoglobulin Heavy Chains HeLa Cells |
| Zdroj: | The Journal of biological chemistry. 265(4) |
| ISSN: | 0021-9258 |
| Popis: | Human rhinoviruses are the major causative agents of the common cold in humans and have been divided into major and minor groups based on receptor specificity. cDNAs encoding the light and heavy chains of a murine monoclonal antibody that recognizes the major group receptor were cloned, abundantly expressed in Escherichia coli, and renatured into Fab fragments that blocked virus binding and protected HeLa cell monolayers from rhinovirus infection. Elimination of the cysteines normally bridging the heavy and light chains yielded molecules indistinguishable from wild-type Fab fragments in virus binding assays. Single-chain antibodies with covalently linked light and heavy variable domains were also expressed and showed receptor binding and cell protection activities. These recombinant antibody fragments are potentially useful in preventing or treating common colds in humans. |
| Databáze: | OpenAIRE |
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