| Autor: |
Josiane, Saadé, Michael, Biacchi, Jérémie, Giorgetti, Antony, Lechner, Alain, Beck, Emmanuelle, Leize-Wagner, Yannis-Nicolas, François |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 2271 |
| ISSN: |
1940-6029 |
| Popis: |
Glycosylation is a crucial posttranslational modification (PTM) that might affect the safety and efficacy of monoclonal antibodies (mAbs). Capillary electrophoresis-mass spectrometry (CE-MS) enables the characterization of the primary structure of mAbs. A bottom-up proteomic workflow is designed to provide detailed information about the glycosylation. In this chapter, we describe the validated experimental protocol applied for the characterization and relative quantification of mAbs N-glycosylation at the glycopeptide level. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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