Purification of a thermostable DNA polymerase from Thermus thermophilus HB8, useful in the polymerase chain reaction

Autor:	N, Carballeira, M, Nazabal, J, Brito, O, Garcia
Rok vydání:	1990
Předmět:	Molecular Weight Kinetics Hot Temperature Base Sequence Drug Stability Molecular Sequence Data DNA DNA-Directed DNA Polymerase Thermus Polymerase Chain Reaction Biotechnology
Zdroj:	BioTechniques. 9(3)
ISSN:	0736-6205
Popis:	A thermostable DNA polymerase, isolated from the thermophilic strain Thermus thermophilus HB 8 was purified by a five-step procedure which provides a high yield and a homogeneous preparation. The molecular weight was estimated to be 67,000 daltons and the extension rate was determined to be 1500 nucleotides per minute. The enzyme works in polymerase chain reaction conditions similar to those used for Taq polymerase from Thermus aquaticus.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::ac181df9f697251d4865f0d1e870ea23 https://pubmed.ncbi.nlm.nih.gov/2223065 Zobrazit plný text záznamu