[Expression of cloned bialaphos resistance gene (bar) in Streptomyces strains]

Autor:	G V, Sezonov, V Iu, Tabakov, E A, Kudriashova
Rok vydání:	1990
Předmět:	Organophosphorus Compounds Genes Bacterial Herbicides R Factors Drug Resistance Microbial Gene Expression Regulation Bacterial Microbial Sensitivity Tests In Vitro Techniques Streptomyces
Zdroj:	Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic]. 35(12)
ISSN:	0235-2990
Popis:	The object of the study was a strain of Streptomyces hygroscopicus producing bialaphos, an antibiotic used as a herbicide, which is promising and ecologically safe. Molecular cloning of a bialaphos resistance gene (bar) was performed in the recipient strain, S. lividans TK64, within the 2.0-kb DNA fragment with the plasmid pIJ699. Introduction of a bar gene into another strain producing bialaphos, i.e. S. viridochromogenus Tu494, led to its higher constitutive resistance to bialaphos. The results confirmed the data on different regulation of bar (S. hygroscopicus) and pat (S. virido-chromogenus) resistance genes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::abfa2e92f398fcb0d5973abd0b7fdbc3 https://pubmed.ncbi.nlm.nih.gov/2078072 Zobrazit plný text záznamu