A spontaneous murine melanoma lung metastasis comprised of host x tumor hybrids
| Autor: | A K, Chakraborty, S, Sodi, M, Rachkovsky, N, Kolesnikova, J T, Platt, J L, Bolognia, J M, Pawelek |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Hypoxanthine
Mice Inbred BALB C Lung Neoplasms Membrane Glycoproteins Time Factors Monophenol Monooxygenase Chemotaxis Immunoblotting Lysosome-Associated Membrane Glycoproteins Sequence Analysis DNA Flow Cytometry Aminopterin Anti-Bacterial Agents Mice Microscopy Electron Antigens CD Cell Movement Antineoplastic Combined Chemotherapy Protocols Tumor Cells Cultured Animals Gentamicins Melanoma Neoplasm Transplantation Polymorphism Restriction Fragment Length Thymidine |
| Zdroj: | Cancer research. 60(9) |
| ISSN: | 0008-5472 |
| Popis: | Cells from a lung metastasis, arising from Cloudman S91 melanoma cells implanted s.c. in the tail of a BALB/c nu/nu mouse, were comprised chiefly of host x tumor hybrids. These lung metastasis cells showed: (a) 30-40% increased DNA content; (b) resistance to 10(-4) M hypoxanthine, 4 x 10(-7) M aminopterin, and 1.6 x 10(-5) M thymidine (HAT) + G418; and (c) the presence in genomic DNA of genes for both wt and albino tyrosinase, reflecting the DBA/2J (Cloudman S91) and BALB/c mouse genotypes, respectively. Individual clones of lung metastasis cells expressed enhanced pigmentation, motility, and responsiveness to MSH/IBMX, a behavior similar to that recently reported for artificially generated melanoma x macrophage fusion hybrids. These similarities suggested that the host fusion partner generating the lung metastasis hybrids might have been a macrophage, although formal proof for this was not possible. The results provide the first direct evidence that host x tumor hybridization could serve as an initiating mechanism for melanoma metastasis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|