The direct demonstration of binding of mu, delta and kappa agonists to digitonin-solubilized opioid receptors from bovine striatum

Autor:	G, Crema, T L, Gioannini, J M, Hiller, E J, Simon
Rok vydání:	1986
Předmět:	Analgesics Receptors Opioid kappa Cell Membrane Receptors Opioid mu Enkephalins Enkephalin Ala(2)-MePhe(4)-Gly(5) Corpus Striatum Benzomorphans Kinetics Morphinans Receptors Opioid delta Receptors Opioid Animals Cattle Oligopeptides Enkephalin Leucine
Zdroj:	NIDA research monograph. 75
ISSN:	1046-9516
Popis:	Active opioid binding sites, that retain the ability to bind tritiated agonists have been obtained in good yield in digitonin/NaCl/Mg2+ extracts of morphine protected bovine striatal membranes. Ligand protection of binding sites and the presence of Mg ions were found to be absolute requirements for agonist binding in this solubilized opioid receptor preparation. Soluble preparations contained a ratio of mu: delta:kappa similar to that in the membranes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::abb2975f61100f34d074565bd413598e https://pubmed.ncbi.nlm.nih.gov/2829015 Zobrazit plný text záznamu