Functional evidence of novel tumor suppressor genes for cutaneous malignant melanoma
| Autor: | C N, Parris, J D, Harris, D K, Griffin, A P, Cuthbert, A J, Silver, R F, Newbold |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Skin Neoplasms
Chromosomes Human Pair 10 Genes p16 Tumor Suppressor Proteins Chromosome Mapping Mice Nude Cell Cycle Proteins Agar Mice Phenotype Neoplastic Stem Cells Tumor Cells Cultured Animals Humans Female Genes Tumor Suppressor Chromosome Deletion Cloning Molecular Carrier Proteins Chromosomes Human Pair 9 Melanoma Cell Division Cyclin-Dependent Kinase Inhibitor p16 Cyclin-Dependent Kinase Inhibitor p15 |
| Zdroj: | Cancer research. 59(3) |
| ISSN: | 0008-5472 |
| Popis: | Losses of heterozygosity involving chromosomes 9 and 10 are frequent events in the development and progression of cutaneous malignant melanoma. To investigate whether specifically deleted chromosomal regions encode tumor suppressor genes (TSGs), we introduced normal chromosome 10 into the tumorigenic human metastatic melanoma cell line UACC-903 by microcell fusion. In addition, two chromosome 9 derivatives that were microdeleted in the region of the p16INK4A/p15INK4B locus were transferred to determine whether an additional melanoma TSG or TSGs reside on chromosome 9p, as indicated by previous melanoma allele loss studies. In comparison to parental cells, microcell hybrids generated with chromosomes 9 (microdeleted) and 10 displayed reduced anchorage-independent growth in soft agar and markedly reduced tumorigenicity in athymic (nu/nu) mice. These data define a TSG or TSGs that function independently of p15/p16 on chromosome 9 and provide evidence for a TSG (or TSGs) on chromosome 10 that may be important in melanoma development. |
| Databáze: | OpenAIRE |
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