Freeze-drying fungi using a shelf freeze-drier

Autor: C Shu-hui, Tan, Cor W, van Ingen, Joost A, Stalpers
Rok vydání: 2007
Předmět:
Zdroj: Methods in molecular biology (Clifton, N.J.). 368
ISSN: 1064-3745
Popis: Lyophilization, the removal of water by freezing and then volatilization at low pressure and temperature, has been employed as a standard long-term preservation method for many filamentous fungi. The method outlined involves the use of standard shelf freeze-drying and skimmed milk as a suspending solution/lyoprotectant. This approach has been employed to freeze-dry the majority of the 50,000 fungal strains that have been successfully lyophilized at the Centraal bureau voor Schimmelcultures (CBS) culture collection (http://www.cbs.knaw.nl/).
Databáze: OpenAIRE