| Autor: |
D M, Desiderio, U, Wirth, J L, Lovelace, G, Fridland, E S, Umstot, T M, Nguyen, P W, Schiller, H S, Szeto, J F, Clapp |
| Rok vydání: |
2000 |
| Předmět: |
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| Zdroj: |
Journal of mass spectrometry : JMS. 35(6) |
| ISSN: |
1076-5174 |
| Popis: |
The synthetic opioid peptide analog Tyr-D-Ala-Gly-N-methyl-Phe-Gly-ol (DAMGO), which is a mu opioid receptor-selective agonist, was quantified in ovine plasma samples with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS), using delayed extraction and a reflectron. The internal standard was pentadeuterated DAMGO. Timed-ion selection was used to select the precursor ion. The analysis of the post-source decay fragments improved the detection sensitivity, and the use of the precursor-product ion relationship optimized the specificity. For plasma samples, the inter-assay variability of this method was 6.4% (n = 79) and the intra-assay variability was 6.0% (n = 10). The variability for controls was 3.4% (n = 43). The profile of DAMGO amount versus time was determined in sheep plasma, and the corresponding pharmacokinetic data were calculated. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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