Functional analysis of yeast gene families involved in metabolism of vitamins B1 and B6

Autor:	Susana, Rodríguez-Navarro, Bertrand, Llorente, María Teresa, Rodríguez-Manzaneque, Anna, Ramne, Genoveva, Uber, Denis, Marchesan, Bernard, Dujon, Enrique, Herrero, Per, Sunnerhagen, José E, Pérez-Ortín
Rok vydání:	2002
Předmět:	Pyridoxal Saccharomyces cerevisiae Proteins Gene Expression Regulation Fungal Multigene Family Two-Hybrid System Techniques Vitamin K 3 RNA Fungal RNA Messenger Saccharomyces cerevisiae Thiamine Blotting Northern Vitamin B 6 Culture Media
Zdroj:	Yeast (Chichester, England). 19(14)
ISSN:	0749-503X
Popis:	In order to clarify their physiological functions, we have undertaken a characterization of the three-membered gene families SNZ1-3 and SNO1-3. In media lacking vitamin B(6), SNZ1 and SNO1 were both required for growth in certain conditions, but neither SNZ2, SNZ3, SNO2 nor SNO3 were required. Copies 2 and 3 of the gene products have, in spite of their extremely close sequence similarity, slightly different functions in the cell. We have also found that copies 2 and 3 are activated by the lack of thiamine and that the Snz proteins physically interact with the thiamine biosynthesis Thi5 protein family. Whereas copy 1 is required for conditions in which B(6) is essential for growth, copies 2 and 3 seem more related with B(1) biosynthesis during the exponential phase.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::aa6b2a8ce1f6bc800fedf203f38c5659 https://pubmed.ncbi.nlm.nih.gov/12271461 Zobrazit plný text záznamu Plný text