A Head-Mounted Camera System Integrates Detailed Behavioral Monitoring with Multichannel Electrophysiology in Freely Moving Mice

Autor:	Arne F, Meyer, Jasper, Poort, John, O'Keefe, Maneesh, Sahani, Jennifer F, Linden
Rok vydání:	2018
Předmět:	Male head movement vestibular Behavior Animal Movement Video Recording oculomotor pupil Article Electrophysiology Mice Inbred C57BL Mice natural behavior Head Movements Vibrissae pinna Image Processing Computer-Assisted Animals whisker visual cortex Eye Movement Measurements Head eye movement
Zdroj:	Neuron
ISSN:	1097-4199
Popis:	Summary Breakthroughs in understanding the neural basis of natural behavior require neural recording and intervention to be paired with high-fidelity multimodal behavioral monitoring. An extensive genetic toolkit for neural circuit dissection, and well-developed neural recording technology, make the mouse a powerful model organism for systems neuroscience. However, most methods for high-bandwidth acquisition of behavioral data in mice rely upon fixed-position cameras and other off-animal devices, complicating the monitoring of animals freely engaged in natural behaviors. Here, we report the development of a lightweight head-mounted camera system combined with head-movement sensors to simultaneously monitor eye position, pupil dilation, whisking, and pinna movements along with head motion in unrestrained, freely behaving mice. The power of the combined technology is demonstrated by observations linking eye position to head orientation; whisking to non-tactile stimulation; and, in electrophysiological experiments, visual cortical activity to volitional head movements. Highlights • Eyes, whiskers, head, and neural activity monitored in freely moving mice • System generates stable video output and leaves mouse behavior largely unchanged • Close link between eye and head movements at both slow and fast timescales • Active head movements in the dark strongly modulate primary visual cortex activity Meyer et al. describe a new head-mounted camera system that enables detailed behavioral monitoring along with neural recording in freely moving mice. The system reveals close coupling between eye and head movements, and head-movement-related activity in primary visual cortex.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::aa5d0830fba6b1f41570fa7fba26ce0f https://pubmed.ncbi.nlm.nih.gov/30308171 Zobrazit plný text záznamu