| Autor: |
Roberta, Mancini, Tobias, van der Bijl, Quentin, Bourgeois-Jaarsma, Rizky, Lasabuda, Alexander J, Groffen |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
Scientific Reports |
| ISSN: |
2045-2322 |
| Popis: |
Recent advances in live Ca2+ imaging with increasing spatial and temporal resolution offer unprecedented opportunities, but also generate an unmet need for data processing. Here we developed SICT, a MATLAB program that automatically identifies rapid Ca2+ rises in time-lapse movies with low signal-to-noise ratios, using fluorescent indicators. A graphical user interface allows visual inspection of automatically detected events, reducing manual labour to less than 10% while maintaining quality control. The detection performance was tested using synthetic data with various signal-to-noise ratios. The event inspection phase was evaluated by four human observers. Reliability of the method was demonstrated in a direct comparison between manual and SICT-aided analysis. As a test case in cultured neurons, SICT detected an increase in frequency and duration of spontaneous Ca2+ transients in the presence of caffeine. This new method speeds up the analysis of elementary Ca2+ transients. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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