Haemopoietic chimaerism: a complication in heterozygote detection tests for inherited defects in cattle

Autor:	P J, Healy, J A, Dennis, P J, Nicholls, K G, Reichmann
Rok vydání:	1994
Předmět:	Male Genotype Chimera Glycogen Storage Disease Type II Genetic Carrier Screening Homozygote Twins Cattle Diseases DNA Fibroblasts Bone Marrow alpha-Mannosidase Mannosidases Animals Citrulline Cattle Female Cells Cultured
Zdroj:	Animal genetics. 25(1)
ISSN:	0268-9146
Popis:	Several observations are documented which illustrate that haemopoietic chimaerism is a potential source of error when using assays of cellular components of blood to determine genotype for inherited defects in cattle. Acidic alpha-glucosidase activity in peripheral mononuclear cells of a twin Brahman bull that had sired calves affected with generalized glycogenosis was similar to that in cells from homozygous normal animals. Activity in fibroblasts from this bull was similar to that in heterozygotes. alpha-mannosidase activity in fibroblasts of a twin Murray Grey bull with low activity in peripheral granulocytes but high activity in plasma was similar to that in animals homozygous normal for alpha-mannosidosis. Normal argininosuccinate synthetase nucleotide sequence was detected in leucocytes from two calves affected with citrullinemia and mutant sequence detected in leucocytes from their homozygous normal co-twins.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::aa156ba49806092e2392ecfbc207ac97 https://pubmed.ncbi.nlm.nih.gov/8161014 Zobrazit plný text záznamu Plný text